Question

Suppose DNA samples collected for DNA fingerprinting analysis are less than the required quantity. Which of the following techniques is helpful to make the samples sufficient for above analysis?

• A. Electrophoresis
• B. Chromatography
• C. PCR
• D. DNA probing

Answer 1

The Correct Option is C

PCR (Polymerase Chain Reaction) is a technique used to amplify (make millions of copies of) a small amount of DNA, making it sufficient for analysis like DNA fingerprinting.

• (A) Electrophoresis: Separates DNA fragments by size but does not amplify DNA.
• (B) Chromatography: Separates molecules based on chemical properties but cannot replicate DNA.
• (D) DNA probing: Identifies specific DNA sequences using labeled probes but does not increase DNA quantity.

Thus, PCR (C) is the only technique that solves the problem of insufficient DNA samples.

Answer 2

PCR (Polymerase Chain Reaction): Amplifies tiny DNA samples exponentially.

Other techniques:

• (A) Electrophoresis: Separates DNA by size.
• (B) Chromatography: Separates molecules by affinity.
• (D) DNA probing: Detects specific sequences.

The correct answer is (C) PCR.