Question

Southern blotting is used to detect

• A. RNA sequences
• B. Protein molecules
• C. Specific DNA sequences
• D. Lipids

Hint:

Remember the blotting techniques: \[ \boxed{ \begin{aligned} \text{Southern blot} &\rightarrow \text{DNA} \text{Northern blot} &\rightarrow \text{RNA} \text{Western blot} &\rightarrow \text{Protein} \end{aligned} } \]

Answer 1

The Correct Option is C

Step 1: Recall the purpose of Southern blotting. Southern blotting involves the transfer of DNA fragments from a gel onto a membrane followed by hybridization with a labelled DNA probe.

Step 2: Identify what it detects. Since complementary probes bind only to matching DNA fragments, \[ \boxed{\text{Southern blotting detects specific DNA sequences}.} \] Therefore, \[ \boxed{(C)} \] is the correct answer.