Phospho-mimic mutants of proteins can be generated by replacing
Show Hint
- serine with aspartic acid
- alanine with glutamic acid
- serine with alanine
- threonine with glutamic acid
The Correct Option is A, D
Solution and Explanation
Step 1: Understanding phospho-mimic mutations.
Phospho-mimic mutations are substitutions that mimic the effect of phosphorylation. The most common phospho-mimic substitutions are replacing serine, threonine, or tyrosine with negatively charged amino acids like aspartic acid or glutamic acid. This substitution mimics the negative charge introduced by phosphorylation.
Step 2: Analyzing the options.
(A) serine with aspartic acid: This is correct. Aspartic acid is negatively charged, and substituting serine with aspartic acid mimics the negative charge of a phosphorylated serine.
(B) alanine with glutamic acid: This is incorrect. Glutamic acid is also negatively charged, but alanine does not represent a phosphorylatable amino acid.
(C) serine with alanine: This is incorrect. Alanine is neutral and would not mimic the effect of phosphorylation.
(D) threonine with glutamic acid: This is incorrect. While glutamic acid is negatively charged, threonine can be phosphorylated, and replacing it with glutamic acid does not mimic phosphorylation.
Step 3: Conclusion.
The correct answer is (A) serine with aspartic acid, as aspartic acid substitution best mimics the effects of phosphorylation.
Top IIT JAM BL Biological Science Questions
- One of Koch's postulates states that the suspected causative organism should
- Archaebacteria differ from bacteria in
- Which one of the following, involving transfer of a phosphate group, is an example of substrate-level phosphorylation?
- Lysosomal targeting of proteins involves recognition of
- In vitro, group I introns have the ability to
Top IIT JAM BL Biochemistry Questions
- Which one of the following, involving transfer of a phosphate group, is an example of substrate-level phosphorylation?
- In polypeptide chains, the proline residue is unique in
- Many plasma glycoproteins are protected from uptake and degradation by the hepatocytes in liver due to the presence of a terminal saccharide moiety known as
Which one of the following matches is CORRECT between the inhibitors given in Group A with their modes of action in Group B?
\[\begin{array}{|c|c|} \hline Group A & Group B \\ \hline \text{(P) Antimycin A} & \text{(i) Inhibits cytochrome c oxidase} \\ \hline \text{(Q) Amytal} & \text{(ii) Blocks electron transfer from cyt b to cyt c1} \\ \hline \text{(R) Carbon monoxide} & \text{(iii) Inhibits adenine nucleotide translocase} \\ \hline \text{(S) Atractyloside} & \text{(iv) Prevents electron transfer from Fe-S centers of complex 1 to ubiquinone} \\ \hline \end{array}\]
- During synthesis of N-linked glycosylated proteins in mammalian cells, which one of the following compositions of sugars is originally added as a core through dolichol-phosphate precursor?
Top IIT JAM BL Questions
- One of Koch's postulates states that the suspected causative organism should
- Archaebacteria differ from bacteria in
- Which one of the following, involving transfer of a phosphate group, is an example of substrate-level phosphorylation?
- Lysosomal targeting of proteins involves recognition of
- In vitro, group I introns have the ability to