Question

Match the techniques in Group I with their applications in Group II for protein analysis 
     Group I                                                                                Group II 
(P) Nuclear magnetic resonance spectroscopy             (1) Proximity between specific sites 
(Q) Fluorescence resonance energy transfer                 (2) Concentration 
(R) Ultraviolet absorption spectroscopy                        (3) Size 
(S) Dynamic light scattering                                          (4) Structure 
 

• A. P-3, Q-1, R-2, S-4
• B. P-4, Q-1, R-2, S-3
• C. P-4, Q-3, R-1, S-2
• D. P-3, Q-4, R-1, S-2

Hint:

NMR, FRET, UV absorption spectroscopy, and DLS are all powerful techniques for studying proteins, each with its specific application.

Answer 1

The Correct Option is B

Step 1: Understanding the techniques and their applications. 
- Nuclear magnetic resonance spectroscopy (NMR) is used to study the structure of proteins (P-4). 

- Fluorescence resonance energy transfer (FRET) measures proximity between specific sites on the protein (Q-1). 

- Ultraviolet absorption spectroscopy helps determine the concentration of the protein by measuring absorption at specific wavelengths (R-2). 

- Dynamic light scattering (DLS) is used to measure the size of proteins and particles (S-3). 
 

Step 2: Analyzing the options. 
(A) P-3, Q-1, R-2, S-4: Incorrect — This does not match the correct pairings. 
(B) P-4, Q-1, R-2, S-3: Correct — This correctly matches the techniques with their applications. 
(C) P-4, Q-3, R-1, S-2: Incorrect — The pairings do not match. 
(D) P-3, Q-4, R-1, S-2: Incorrect — The pairings are incorrect. 
 

Step 3: Conclusion. 
The correct answer is (B) P-4, Q-1, R-2, S-3.