Question

Match List I with List II
List I
a) Visualization of DNA in gel electrophoresis
b) Precipitation of DNA
c) Breaking of cell wall of bacteria
d) Breaking of cell wall of fungus
List II
i) Chitinase
ii) Lysozyme
iii) Chilled ethanol
iv) Ethidium bromide
Codes:

• A. a - iv, b - iii, c - ii, d - i
• B. a - iii, b - i, c - i, d - ii
• C. a - iv, b - i, c - ii, d - iii
• D. a - iii, b - ii, c - iv, d - i

Hint:

Remember target-enzyme pairs: Bacteria = Lysozyme, Plant = Cellulase, Fungi = Chitinase.

Answer 1

The Correct Option is A

Step 1: Understanding the Concept: Various biochemical agents and enzymes are utilized at different stages of DNA isolation and processing.
Step 2: Key Formula or Approach: Match each specific laboratory technique or biological barrier with its corresponding chemical reagent or degrading enzyme.
Step 3: Detailed Explanation:
(a) To visualize separated DNA fragments in gel electrophoresis, the gel is stained with the fluorescent dye Ethidium bromide (iv) followed by exposure to UV radiation.
(b) During DNA isolation, purified DNA is ultimately precipitated out of the solution by the addition of Chilled ethanol (iii).
(c) The bacterial cell wall, primarily made of peptidoglycan, is degraded using the enzyme Lysozyme (ii) to release the cellular contents.
(d) The fungal cell wall, which is composed of chitin, requires the enzyme Chitinase (i) to break it open.
Therefore, the correct matching sequence is a-iv, b-iii, c-ii, d-i.
Step 4: Final Answer: The correct sequence corresponds to option (1).