Question:
Liquid chromatography can be performed in which of the following ways?
Liquid chromatography can be performed in which of the following ways?
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To remember this, think of the two "extremes": HPLC uses a metal Column for complex industrial analysis, while a simple lab student might use a flat Paper strip for a basic ink separation. Both are forms of liquid chromatography!
Updated On: Jul 1, 2026
- Only in columns
- Only on plane surfaces
- Either in columns or on plane surfaces
- Neither in columns nor on plane surfaces
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The Correct Option is C
Solution and Explanation
Liquid chromatography (LC) is a versatile separation technique where the mobile phase is a liquid. It is classified into different types based on the physical arrangement of the stationary phase.
1. Column Chromatography:
The most common form is column chromatography, where the stationary phase is packed into a cylindrical tube (column). The mobile phase flows through this column under gravity or high pressure (as in HPLC). This method is highly effective for both analytical and preparative separations.
2. Planar Chromatography: Liquid chromatography can also be performed on a flat, "plane" surface.
• Paper Chromatography: The stationary phase is the water molecules trapped in the cellulose fibers of the paper.
• Thin-Layer Chromatography (TLC): The stationary phase is a thin layer of adsorbent material (like silica gel) coated onto a glass, plastic, or aluminum plate.
3. Conclusion: Because liquid chromatography encompasses both high-pressure column systems and simple planar techniques like TLC and paper chromatography, it is performed in both formats. Therefore, it can be conducted either in columns or on plane surfaces.
2. Planar Chromatography: Liquid chromatography can also be performed on a flat, "plane" surface.
• Paper Chromatography: The stationary phase is the water molecules trapped in the cellulose fibers of the paper.
• Thin-Layer Chromatography (TLC): The stationary phase is a thin layer of adsorbent material (like silica gel) coated onto a glass, plastic, or aluminum plate.
3. Conclusion: Because liquid chromatography encompasses both high-pressure column systems and simple planar techniques like TLC and paper chromatography, it is performed in both formats. Therefore, it can be conducted either in columns or on plane surfaces.
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