Question

Intraoperatively, which stain is used to view the following lesion:

• A. AgNO3
• B. Toluidine Blue
• C. Congo red
• D. Methylene blue

Hint:

Toluidine blue is widely used for identifying epithelial lesions during surgeries, especially in procedures involving the oral cavity or urogenital tract.

Answer 1

The Correct Option is B

In order to determine which stain is used intraoperatively to view the lesion in question, we need to understand the applications and characteristics of the given stains:

1. AgNO₃ (Silver Nitrate): Primarily used in histology for staining nerve cells or similar tissues due to its reaction with certain proteins and tissue structures.
2. Toluidine Blue: A metachromatic stain commonly used to highlight acidic tissue components, such as proteoglycans in cartilage, and is often used in surgeries to detect mucopolysaccharides which are components of connective tissue lesions.
3. Congo Red: Typically used for amyloid staining because it binds to the fibrillar amyloid proteins, exhibiting apple-green birefringence under polarized light.
4. Methylene Blue: Used as a general counterstain and for highlighting cellular components, prominently used in microbiology labs rather than for specific tissue or lesion identification in surgeries.

Given these functionalities, Toluidine Blue is the appropriate choice for intraoperative staining of lesions as it allows for the clear differentiation of tissue structures, especially in contexts involving connective tissue.