Question

Insertional inactivation is commonly used for screening recombinants in

• A. YAC vectors
• B. BAC vectors
• C. Cosmid vectors
• D. pBR322 plasmid

Hint:

Common screening methods: \[ \boxed{ \begin{aligned} \text{pBR322} &\rightarrow \text{Insertional inactivation} \text{pUC vectors} &\rightarrow \text{Blue-white screening} \end{aligned} } \]

Answer 1

The Correct Option is D

Step 1: Recall insertional inactivation. Insertional inactivation is a method of identifying recombinant DNA by inserting foreign DNA into a marker gene, thereby disrupting its normal function.

Step 2: Identify the appropriate vector. The plasmid pBR322 contains antibiotic resistance genes (Amp\(^R\) and Tet\(^R\)). Insertion of foreign DNA into one of these genes inactivates it, allowing recombinant colonies to be distinguished from non-recombinants. Hence, \[ \boxed{\text{pBR322 plasmid}} \] is the correct answer. Therefore, \[ \boxed{(D)} \] is the correct answer.