Using the relations between roots,
\[
\alpha+\beta=-\frac{b}{a},\qquad
\alpha\beta=\frac{c}{a}.
\]
Now,
\[
\left(\frac{\alpha}{\beta}-\frac{\beta}{\alpha}\right)^2
=
\frac{(\alpha^2-\beta^2)^2}{(\alpha\beta)^2}.
\]
Since
\[
\alpha^2-\beta^2=(\alpha+\beta)(\alpha-\beta),
\]
and
\[
(\alpha-\beta)^2
=(\alpha+\beta)^2-4\alpha\beta
=
\frac{b^2-4ac}{a^2},
\]
therefore,
\[
(\alpha^2-\beta^2)^2
=
\frac{b^2(b^2-4ac)}{a^4}.
\]
Also,
\[
(\alpha\beta)^2=\frac{c^2}{a^2}.
\]
Hence,
\[
\left(\frac{\alpha}{\beta}-\frac{\beta}{\alpha}\right)^2
=
\frac{b^2(b^2-4ac)}{a^2c^2}.
\]
Therefore,
\[
\boxed{(A)}
\]