Question:
Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :
Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :
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Remember the order D-A-E: Denature (Heat), Anneal (Cool), Extend (Synthesize).
Updated On: May 3, 2026
- Annealing → Denaturation → Extension
- Extension → Annealing → Denaturation
- Denaturation → Extension → Annealing
- Denaturation → Annealing → Extension
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The Correct Option is D
Solution and Explanation
The Polymerase Chain Reaction (PCR) is used to amplify DNA in vitro. Each cycle consists of three steps in a specific thermal sequence:
• Denaturation: Heating the target DNA to approximately $94^\circ\text{C}$ to separate the double-stranded DNA into single strands.
• Annealing: Lowering the temperature to allow primers to bind to their complementary sequences on the single-stranded DNA.
• Extension: The temperature is adjusted (usually to $72^\circ\text{C}$) to allow *Taq* polymerase to synthesize new DNA strands starting from the primers.
• Denaturation: Heating the target DNA to approximately $94^\circ\text{C}$ to separate the double-stranded DNA into single strands.
• Annealing: Lowering the temperature to allow primers to bind to their complementary sequences on the single-stranded DNA.
• Extension: The temperature is adjusted (usually to $72^\circ\text{C}$) to allow *Taq* polymerase to synthesize new DNA strands starting from the primers.
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