In bacterial translation elongation, tRNA translocation is a crucial step where the tRNA moves from the P (peptidyl) site to the E (exit) site of the ribosome. During this process, an elongation factor known as EF-G is required, which facilitates the movement of the ribosome along the mRNA. This translocation is powered by the hydrolysis of a GTP molecule. Therefore, each translocation event consumes exactly one GTP molecule.