Question

How does fed-batch fermentation help in overcoming substrate inhibition?

• A. By maintaining a high substrate concentration
• B. By maintaining a low substrate concentration
• C. By adding substrate in a single batch
• D. By not adding substrate at all

Hint:

Fed-batch fermentation: \[ \boxed{ \text{Gradual substrate addition} \rightarrow \text{Low substrate concentration} \rightarrow \text{Reduced substrate inhibition} } \]

Answer 1

The Correct Option is B

Step 1: Recall the principle of fed-batch fermentation. In fed-batch fermentation, fresh substrate is added gradually instead of all at once.

Step 2: Identify how substrate inhibition is prevented. Continuous or controlled feeding prevents the substrate from accumulating to inhibitory levels. Thus, the substrate concentration is maintained at a low optimum level. Hence, \[ \boxed{\text{By maintaining a low substrate concentration}} \] is the correct answer. Therefore, \[ \boxed{(B)} \] is the correct answer.