DNA finger printing technique was first developed by________
- Schleiden and Schwan
- Edwards and Steptoe
- Jeffreys, Wilson and Thien
- Boysen and Jensen
The Correct Option is C
Solution and Explanation
Top KCET Biology Questions
- Relate Column I with Column II with regard to predatory behaviour:
Column I Column II 1. Calotropis p. Invertebrates 2. Pisaster q. Distasteful 3. Monarch butterfly r. Cryptically colored 4. Frogs s. Cardioglycoside - Small mammals and birds are rarely found in polar regions. The reason is that
Match Column I and Column I
Column I Column II 1 Narrowly utilitarian argument p Conserving biodiversity for major ecosystem services 2 Broadly utilitarian argument q Every species has an intrinsic value and moral duty to pass our biological legacy in good order to future generation. 3 Ethical argument r Receiving benefits like food, medicine & industrial products. - Identify the correct statement/s about ex situ conservation. Advanced ex situ conservation includes i. Cryopreservation of gametes. ii. Plant tissue culture method. iii. Seed bank. iv. In vitro fertilisation.
- Identify the odd one out.
Top KCET DNA fingerprinting Questions
Which aspect forms the basis of DNA finger- printing?
- Arrange the below given steps of DNA fingerprinting in sequence by selecting the appropriate option.
a) Digestion of DNA by restriction endonucleases
b) Autoradiography
c) Blotting of DNA fragments to nitrocellulose membrane
d) Isolation of DNA
e) Separation of DNA fragments by electrophoresis
Top KCET Questions
- Which of the following is not true for oxidation?
- If $a, b$ and $c \in N$ which one of the following is not true ?
- When $50\, cm ^{3}$ of $0.2\, N$ $H_{2} SO _{4}$ is mixed with $50 cm ^{3}$ of $1 N\, KOH$, the heat liberated is :
- If $a | (b + c)$ and $a| (b-c)$ where $a,b,c \in \, N $ then
- Which of the following is NOT a pair of functional isomers?
Concepts Used:
DNA Fingerprinting
DNA fingerprinting, also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics, method of isolating and identifying variable elements within the base-pair sequence of DNA.
The procedure of DNA Fingerprinting:
The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes. The enzymes produced fragments of varying lengths that were sorted by placing them on a gel and then subjecting the gel to an electric current (electrophoresis): the shorter the fragment, the more quickly it moved toward the positive pole (anode). The sorted double-stranded DNA fragments were then subjected to a blotting technique in which they were split into single strands and transferred to a nylon sheet. The fragments underwent autoradiography in which they were exposed to DNA probes—pieces of synthetic DNA that were made radioactive and that bound to the minisatellites. A piece of X-ray film was then exposed to the fragments, and a dark mark was produced at any point where a radioactive probe had become attached. The resultant pattern of marks could then be analyzed.