Question

Arrange the following steps of DNA fingerprinting in a correct sequence.
A. Isolation of DNA and its digestion by restriction endonucleases.
B. Hybridisation using labelled VNTR probe.
C. Transferring of separated DNA fragments to synthetic membranes.
D. Detection of hybridised DNA fragments by autoradiography.
E. Separation of DNA fragments by electrophoresis. Choose the correct answer from the options given below:

• A. A, B, D, C, E
• B. A, E, C, B, D
• C. A, D, B, E, C
• D. A, E, B, C, D

Hint:

The process flow: \textbf{Cut} (A) $\rightarrow$ \textbf{Separate} (E) $\rightarrow$ \textbf{Transfer} (C) $\rightarrow$ \textbf{Probe} (B) $\rightarrow$ \textbf{Detect} (D).

Answer 1

The Correct Option is B

DNA fingerprinting involves a specific sequential protocol to identify individuals based on their unique DNA patterns:
• Isolation and Digestion (A): DNA is extracted and cut into pieces using restriction enzymes.
• Electrophoresis (E): The DNA fragments are separated based on size.
• Blotting (C): The separated DNA fragments are transferred (blotted) onto a synthetic membrane (Nitrocellulose or Nylon).
• Hybridisation (B): The membrane is treated with a labeled VNTR (Variable Number Tandem Repeat) probe.
• Detection (D): The hybridised DNA is visualized using autoradiography.