Step 1: Relation between electric field and potential.
\[
E = -\frac{dV}{dx}
\]
Step 2: Express potential difference.
\[
V_0 - V_4 = \int_{0}^{4} E \, dx
\]
Step 3: Substitute given electric field.
\[
V_0 - V_4 = \int_{0}^{4} 3x^2 \, dx
\]
Step 4: Perform integration.
\[
\int 3x^2 dx = x^3
\]
Step 5: Apply limits.
\[
V_0 - V_4 = \left[ x^3 \right]_{0}^{4} = 4^3 - 0 = 64
\]
Step 6: Final conclusion.
\[
\boxed{64 \, \text{V}}
\]
Hence, correct answer is option (D).