Question

A sample of glucose isomerase enzyme converts 15 \(\mu\)moles of substrate glucose into fructose per min per mL under standard assay conditions. The enzyme activity in International Unit (IU) is ................ (in integer).

Hint:

Remember: 1 IU = 1 \(\mu\)mol of substrate converted per minute under standard conditions. So here the numerical value in \(\mu\)mol/min directly equals the IU.

Answer 1

Definition: 1 International Unit (IU) = the amount of enzyme that catalyzes the conversion of 1 \(\mu\)mol of substrate per min under standard assay conditions.
Here, rate of conversion = 15 \(\mu\)mol/min/mL.
\(⇒\) Enzyme activity = \(15 \ \text{IU/mL}\).
\fbox{Enzyme activity = 15 IU}