Question

A major limitation of RAPD is

• A. Low sensitivity
• B. Poor reproducibility
• C. Requirement of radioactive probes
• D. High cost

Hint:

RAPD characteristics: \[ \boxed{ \begin{aligned} &\text{PCR-based marker} &\text{No prior sequence information required} &\text{Major limitation: Poor reproducibility} \end{aligned} } \]

Answer 1

The Correct Option is B

Step 1: Recall RAPD. RAPD (Random Amplified Polymorphic DNA) is a PCR-based technique that uses short arbitrary primers to amplify random DNA segments.

Step 2: Identify its limitation. Because RAPD is highly sensitive to PCR conditions such as DNA quality, primer concentration and annealing temperature, its results are often difficult to reproduce. Thus, \[ \boxed{\text{Poor reproducibility}} \] is the major limitation of RAPD. Therefore, \[ \boxed{(B)} \] is the correct answer.