Question

Which of the following PCR is used to amplify an unknown sequence of DNA if only a single sequence is known?

• A. Anchored PCR
• B. Touchdown PCR
• C. Reverse transcription PCR (RT-PCR)
• D. Hotstart PCR

Hint:

Anchored PCR: for unknown sequences.
Uses one known primer and one degenerate primer.
Also called RACE PCR.

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
Different types of PCR are used for different purposes.
Some are used for amplifying unknown sequences.

Step 2: Key Formula or Approach:
No specific formula.
Need to know the types of PCR.

Step 3: Detailed Explanation:
Anchored PCR is used when only a single sequence is known.
It uses a known sequence to anchor the primer.
Touchdown PCR is for improving specificity.
RT-PCR is for RNA.
Hotstart PCR is for preventing non-specific amplification.
Thus, Anchored PCR is used for unknown sequences.

Step 4: Final Answer:
Anchored PCR is used to amplify an unknown sequence.
Hence, the correct option is (A).