Question:

Which of the following diluent used in Swab and rinse method to check the hygiene status of dairy equipment?

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Always associate the standard sanitation testing of dairy equipment (swab/rinse methods) with quarter-strength Ringer's solution as the preferred, non-nutritive, isotonic diluent.
  • Normal saline
  • Peptone water
  • Half strength ringers solution
  • Quarter strength ringers solution
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The Correct Option is D

Solution and Explanation

Step 1: Understanding the Concept:
Evaluating the sanitation and hygiene of dairy processing equipment requires using a swab or rinse diluent that maintains the viability of collected microorganisms without allowing them to multiply before plating.

Step 2: Detailed Explanation:

- Quarter-strength Ringer's solution is an isotonic salt solution that maintains osmotic balance, protecting bacterial cells from osmotic shock or lysis.
- Because it contains no organic nutrients, it prevents bacteria from multiplying during transport to the laboratory, ensuring an accurate count of the original contamination level.
- While normal saline or peptone water can be used in some general microbiological applications, quarter-strength Ringer's solution is the standard recommended diluent for swab and rinse tests in the dairy industry.

Step 3: Final Answer

The diluent commonly used in swab and rinse sanitation tests is quarter-strength Ringer's solution.
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