Question:
In a radioimmunoassay (RIA), the amount of unlabelled antigen is inversely proportional to:
In a radioimmunoassay (RIA), the amount of unlabelled antigen is inversely proportional to:
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RIA: competitive binding assay.
Inverse relationship: more unlabelled antigen = less signal.
Used to measure hormones, drugs.
Inverse relationship: more unlabelled antigen = less signal.
Used to measure hormones, drugs.
Updated On: Aug 18, 2026
- IgG antibodies
- IgM antibodies
- Antigen concentration
- IgE antibodies
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The Correct Option is C
Solution and Explanation
Step 1: Understanding the Concept:
Radioimmunoassay is a competitive binding assay.
It measures the amount of unlabelled antigen.
Step 2: Key Formula or Approach:
No specific formula.
Need to understand the principle of RIA.
Step 3: Detailed Explanation:
In RIA, there is a competition between labelled and unlabelled antigen.
The amount of unlabelled antigen is inversely proportional to the signal.
The signal is from the bound labelled antigen.
Higher unlabelled antigen means less labelled antigen bound.
Thus, the amount of unlabelled antigen is inversely proportional to antigen concentration.
Step 4: Final Answer:
The amount of unlabelled antigen is inversely proportional to antigen concentration.
Hence, the correct option is (C).
Radioimmunoassay is a competitive binding assay.
It measures the amount of unlabelled antigen.
Step 2: Key Formula or Approach:
No specific formula.
Need to understand the principle of RIA.
Step 3: Detailed Explanation:
In RIA, there is a competition between labelled and unlabelled antigen.
The amount of unlabelled antigen is inversely proportional to the signal.
The signal is from the bound labelled antigen.
Higher unlabelled antigen means less labelled antigen bound.
Thus, the amount of unlabelled antigen is inversely proportional to antigen concentration.
Step 4: Final Answer:
The amount of unlabelled antigen is inversely proportional to antigen concentration.
Hence, the correct option is (C).
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